The heart of any HPLC system is the pumping system. The pumping system must provide a steady and consistent flow to give reproducible retention times. Peak shape, as well as, retention times are dependent on the pumping system. The pumps are required to pull solvent from a reservoir at ambient pressure and deliver the liquid to the column under pressures up to 400 bar. This must be done with liquids of varying viscosity. Typically, the pumping system is also required to accurately mix multiple solvents to provide a smooth and reproducible gradient.